The Storyteller was the one withdrawal as the ultimate discipline of 40 horses – headed by Material Cap – was named for the Grand Nationwide on Saturday.
It means Blaklion is the final horse to be assured a run within the large race at Aintree.
Some Neck and Welsh Grand Nationwide winner Secret Reprieve are the highest reserves.
Patrick Mullins will journey main contender Burrows Saint for his coach father Willie Mullins.
Steady jockey Paul Townend will miss the journey after struggling a foot damage.
A reserve could make the line-up if any contenders are pulled out earlier than 13:00 BST on Friday.
Material Cap, educated by Jonjo O’Neill, is the Four-1 favorite to present proprietor Trevor Hemmings a file fourth win in his inexperienced, yellow and white colors.
Mullins is one among 4 newbie jockeys to be booked for rides within the race – with Jamie Codd on Milan Native, Derek O’Connor driving OK Corral and Sam Waley-Cohen aboard Jett.
They are going to be bidding to be the primary newbie rider to triumph since Marcus Armytage on Mr Frisk in 1990 and are permitted to journey after the most recent easing of lockdown restrictions, having been barred from competing finally month’s Cheltenham Pageant.
One contender who appears positive to be standard is Minella Instances, the mount of Rachael Blackmore – who made history at Cheltenham by changing into the primary lady to be main jockey on the assembly.
Bryony Frost is on Yala Enki and Tabitha Worsley rides Sub Lieutenant within the hunt to be the primary feminine jockey to win the Grand Nationwide.
Final yr’s assembly was known as off due to the Covid-19 pandemic, whereas this yr’s contest can be held with out spectators.
Police are stepping up patrols across the assembly, with residents near the Merseyside observe warned to not collect in teams bigger than six of their gardens in the course of the races.